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KMID : 1041219970390010067
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Journal of Sericultural and Entomological Science
1997 Volume.39 No. 1 p.67 ~ p.72
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Dialytic pH Condition for Obtaining Silk Ⅰ Type Structure
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Abstract
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As a condition for obtaining the silk I type crystal that has stability and high reproducibility, after dissolving silk fibroin crystalline part (Fcp), the changes of recrystallized crystal structure according to dialytic pH were examined by x-ray diffraction and differential thermal analysis. The Fcp was obtained from the aqueous solution of silk fibroin enzymatic proteolyzed by chymotrypsin. The crystal structure of Fcp showed silk II type. When Fcp was dissolved by 10M LiBr aqueous solution, the Fcp1 showed silk II type at pH9. However, besides the silk II type, the silk I type structure begins to appear at pH8 and only the silk I type structure was found below pH6. On the other hand, the Fcp2 that calcium chloride was used in the dissolution found only the silk I type crystal structure below pH8.
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